These months I had worked with the hydrocarbon phenantrene. A technique that I learned and realized in the laboratory is how to know the Minimum Inhibitory Concentration (MIC) of the bioluminescent bacteria in this hydrocarbon. First of all, we did many dilutions of phenantrene calculating the concentration of the medium. By this way, we found at what concentration the different bacteria may grow. Then, we inoculated the bacteria in the medium with phenantrene at a specific concentration beginning with an optical density of .025 measured with a spectrophotometer. After 3 hours in the shaker at 37°C, we inoculated 100 micro liters of bacteria in the medium and incubated them for 24 hours. The results shows grow of the bacteria in the medium, but now we have to analyze the absorbencies given in the spectrophotometer after the experiment.
This experiment will be repeated to confirm the results and will be repeated with other organisms. The next step is to see if they can degrade the hydrocarbon. For this reason I think that I had completed 50% of the research objectives. The expectative is positive to me and all the lab team.
Good job!