These last months I had been busy with the class works and tests, but I, always, spend time in what defines me: science investigation. Last time, I wrote that we found at what concentration of the hydrocarbon phenathrene the bioluminescent bacteria may grow doing the test of Minimum Inhibitory Concentration (MIC). Knowing this, we inoculated 6 different organisms in a medium with phenanthrene and incubated them for a week. Then we found that all the organisms grew but when we obtained the optical density in a spectophotomter, this one was too little. The experiment was repeated and the same results were obtained. We can infere many things about this, like the phenanthrene are breaking the membrane of the cells. If this is happening we have to design another experiment with other interesting purpose. For now, we will repeat the experiment with a lower concentration of phenanthrene to see if we obtain different results related with the optical density. I think that I had completed 50%-80% of the experiment objectives. In future plans we will repeat this experiment with the hydrocarbon para-nitrophenol.
[...] Original post by evanelly [...]
Eva!!! Congrats for your results! After all the trouble you have gone through and all the setbacks with the experimental conditions, its great that you have completed most of your objectives. Keep us posted on your latest findings! See you @ Immuno!